HT-29

HT-29是廣泛應用於生物學和癌症研究的人類結腸癌細胞系，最初於1964年從一名44歲的白人女性患者分離出來的^[1]。

特徵

細胞中過度表達的p53基因

HT-29細胞形成緊密的單層膜，同時表現出與小腸上皮細胞的相似性。HT-29細胞過度表達p53基因，但p53基因在273位擁有突變，導致組氨酸取代了精氨酸的位置。HT-29會在含有蘇拉明的培養基中快速增殖，並且具有相應的c-myc癌基因高度表達。c-myc基因若然被解除管制（deregulated），則可能與HT-29細胞的生長因子需求有關^[2]。

科研用途

在臨床前研究中，已經研究了HT-29細胞在體外分化及模擬真實結腸組織的能力。這特性使HT-29細胞可應用於上皮細胞研究^[3]，還可以在體內進行測試。HT-29細胞在細胞培養物中以半乳糖替代葡萄糖的位置，並且添加丁酸酯或酸來進行終末分化，分化為腸上皮細胞，從而可以研究其細胞分化途徑及其對周圍環境的依賴性^[1]。對HT-29細胞的研究表明，歸因於毛喉素、秋水仙素、諾考達唑和紫杉醇誘導的分化^[4]，半乳糖介導的細胞分化會增強黏著小帶^[5]。

培養

儘管HT-29細胞可以在缺乏生長因子的細胞培養基中增殖，但其倍增時間約為4天，而在培養基中添加胎牛血清，可以將HT-29細胞的倍增時間減少至1天^[2]。HT-29細胞具有很高的葡萄糖消耗，並且在含有25 mM葡萄糖和10%血清的標準培養基中並未出現細胞分化^[1]。

參考資料

^ ^1.0 ^1.1 ^1.2 Martínez-Maqueda, D; et al. HT29 Cell Line. The Impact of Food Bioactives on Health. Springer. 2015: 113–124. ISBN 978-3-319-15791-7. PMID 29787047. doi:10.1007/978-3-319-16104-4_11.
^ ^2.0 ^2.1 Coudray, Anne-Marie; et al. Proliferation of the Human Colon Carcinoma Cell Line HT29: Autocrine Growth and Deregulated Expression of the c-myc Oncogene (PDF). Cancer Research. 1 December 1989, 49: 6566–6571 [2020-01-01]. （原始内容存档 (PDF)于2020-01-01）.
^ Hirn, M; et al. HT-29 cells are an in vitro model for the generation of cell polarity in epithelia during embryonic differentiation. Proceedings of the National Academy of Sciences. 1988, 85 (1): 136–140. PMC 279498  . PMID 3277169. doi:10.1073/pnas.85.1.136.
^ Cohen, E; et al. Induced differentiation in HT29, a human colon adenocarcinoma cell line. Journal of Cell Science. August 1999, 112: 2657–2666. PMID 10413674.
^ Gout, S; et al. Early enterocytic differentiation of HT-29 cells: biochemical changes and strength increases of adherens junctions. Experimental Cell Research. 1 October 2004, 299 (1): 498–510. PMID 15350547. doi:10.1016/j.yexcr.2004.06.008.

外部連結

Cellosaurus entry for HT-29（页面存档备份，存于互联网档案馆）

[one-1] 1.0 ^1.1 ^1.2 Martínez-Maqueda, D; et al. HT29 Cell Line. The Impact of Food Bioactives on Health. Springer. 2015: 113–124. ISBN 978-3-319-15791-7. PMID 29787047. doi:10.1007/978-3-319-16104-4_11.

[two-2] 2.0 ^2.1 Coudray, Anne-Marie; et al. Proliferation of the Human Colon Carcinoma Cell Line HT29: Autocrine Growth and Deregulated Expression of the c-myc Oncogene (PDF). Cancer Research. 1 December 1989, 49: 6566–6571 [2020-01-01]. （原始内容存档 (PDF)于2020-01-01）.

[3] Hirn, M; et al. HT-29 cells are an in vitro model for the generation of cell polarity in epithelia during embryonic differentiation. Proceedings of the National Academy of Sciences. 1988, 85 (1): 136–140. PMC 279498  . PMID 3277169. doi:10.1073/pnas.85.1.136.

[4] Cohen, E; et al. Induced differentiation in HT29, a human colon adenocarcinoma cell line. Journal of Cell Science. August 1999, 112: 2657–2666. PMID 10413674.

[5] Gout, S; et al. Early enterocytic differentiation of HT-29 cells: biochemical changes and strength increases of adherens junctions. Experimental Cell Research. 1 October 2004, 299 (1): 498–510. PMID 15350547. doi:10.1016/j.yexcr.2004.06.008.

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