PC-3

PC-3是用于前列腺癌研究和药物开发的人类前列腺癌细胞系，最初是在1979年分离自一名62岁白人男性的前列腺癌患者^[1]。

特征

常见前列腺癌细胞系的特性

PC-3细胞睾丸激素 5α还原酶及酸性磷酸酶的活性低，并且不会表达前列腺特异抗原。有核型分析报告表明PC-3细胞是近三倍体的，具有62条染色体，当中并没有Y染色体的存在。电子显微镜显示PC-3细胞表现出低分化腺癌的特征，并且具有上皮起源的赘生性细胞共有的特征，例如许多微绒毛、接合复合物、异常的细胞核、核仁及线粒体、环孔片层（英语：Annulate lamella）及脂质体。这些细胞对雄激素、糖皮质激素及成纤维细胞生长因子并没有任何反应^[2]，但有研究表明该细胞会受表皮生长因子所影响^[3]。这些细胞可在小鼠中建立皮下肿瘤异种移植物，以研究肿瘤环境和治疗药物功能。PC-3细胞可用于研究晚期前列腺癌细胞的生化变化，以及评估其对化学治疗剂的反应。 PC-3细胞还用于研究表现出免疫反应的哺乳动物细胞中的病毒感染^[4]。与具有中等转移潜能的DU145细胞和具有低转移潜能的LNCaP细胞相比，PC-3细胞具有较高的转移潜能^[5]。

另见

DU145
LNCaP

参考资料

^ Kaighn, ME; Narayan, KS; Ohnuki, Y; Lechner, JF; Jones, LW. Establishment and characterization of a human prostatic carcinoma cell line (PC-3).. Investigative urology. 1979-07, 17 (1): 16–23 [2020-01-13]. PMID 447482. （原始内容存档于2020-01-13）.
^ Tai, S; Sun, Y; Squires, JM; Zhang, H; Oh, WK; Liang, CZ; Huang, J. PC3 is a cell line characteristic of prostatic small cell carcinoma.. The Prostate. 2011-11, 71 (15): 1668–79 [2020-01-13]. PMID 21432867. doi:10.1002/pros.21383. （原始内容存档于2020-01-13）.
^ Johnston, ST; Shah, ET; Chopin, LK; Sean McElwain, DL; Simpson, MJ. Estimating cell diffusivity and cell proliferation rate by interpreting IncuCyte ZOOM™ assay data using the Fisher-Kolmogorov model.. BMC systems biology. 2015-07-19, 9: 38 [2020-01-13]. PMID 26188761. doi:10.1186/s12918-015-0182-y. （原始内容存档于2020-01-13）.
^ Timm, C; Gupta, A; Yin, J. Robust kinetics of an RNA virus: Transcription rates are set by genome levels.. Biotechnology and bioengineering. 2015-08, 112 (8): 1655–62 [2020-01-13]. PMID 25726926. doi:10.1002/bit.25578. （原始内容存档于2020-01-13）.
^ Pulukuri, SM; Gondi, CS; Lakka, SS; Jutla, A; Estes, N; Gujrati, M; Rao, JS. RNA interference-directed knockdown of urokinase plasminogen activator and urokinase plasminogen activator receptor inhibits prostate cancer cell invasion, survival, and tumorigenicity in vivo.. The Journal of biological chemistry. 2005-10-28, 280 (43): 36529–40 [2020-01-13]. PMID 16127174. doi:10.1074/jbc.M503111200. （原始内容存档于2020-01-13）.

外部链接

Cellosaurus entry for PC-3（页面存档备份，存于互联网档案馆）

[1] Kaighn, ME; Narayan, KS; Ohnuki, Y; Lechner, JF; Jones, LW. Establishment and characterization of a human prostatic carcinoma cell line (PC-3).. Investigative urology. 1979-07, 17 (1): 16–23 [2020-01-13]. PMID 447482. （原始内容存档于2020-01-13）.

[a-2] Tai, S; Sun, Y; Squires, JM; Zhang, H; Oh, WK; Liang, CZ; Huang, J. PC3 is a cell line characteristic of prostatic small cell carcinoma.. The Prostate. 2011-11, 71 (15): 1668–79 [2020-01-13]. PMID 21432867. doi:10.1002/pros.21383. （原始内容存档于2020-01-13）.

[3] Johnston, ST; Shah, ET; Chopin, LK; Sean McElwain, DL; Simpson, MJ. Estimating cell diffusivity and cell proliferation rate by interpreting IncuCyte ZOOM™ assay data using the Fisher-Kolmogorov model.. BMC systems biology. 2015-07-19, 9: 38 [2020-01-13]. PMID 26188761. doi:10.1186/s12918-015-0182-y. （原始内容存档于2020-01-13）.

[4] Timm, C; Gupta, A; Yin, J. Robust kinetics of an RNA virus: Transcription rates are set by genome levels.. Biotechnology and bioengineering. 2015-08, 112 (8): 1655–62 [2020-01-13]. PMID 25726926. doi:10.1002/bit.25578. （原始内容存档于2020-01-13）.

[5] Pulukuri, SM; Gondi, CS; Lakka, SS; Jutla, A; Estes, N; Gujrati, M; Rao, JS. RNA interference-directed knockdown of urokinase plasminogen activator and urokinase plasminogen activator receptor inhibits prostate cancer cell invasion, survival, and tumorigenicity in vivo.. The Journal of biological chemistry. 2005-10-28, 280 (43): 36529–40 [2020-01-13]. PMID 16127174. doi:10.1074/jbc.M503111200. （原始内容存档于2020-01-13）.

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