DLD-1

DLD-1是非整數倍體的人類結直腸腺癌細胞系^[1] (Human Dukes’s type C colorectal adenocarcinoma cell line) ，最初是於1977年至1979年間分離出來的兩株結直腸腺癌細胞株之一，對p53蛋白及多種癌基因 (例如C-Myc^[2]、KRAS（英語：KRAS）^[3]、HRAS（英語：HRAS）及NRAS（英語：Neuroblastoma RAS viral oncogene homolog）等^[4]) 呈現陽性反應。有研究指出其擁有較強的爬行和侵襲能力，故而擁有高遷移性與侵襲能力^[5]。

科研用途

2006年，有科研人員發現生育三烯酚（英語：Tocotrienol）以時間和劑量依賴性方式抑制DLD-1細胞的端粒酶活性，其中δ-生育三烯酚抑制其活性的效果最明顯，表明生育三烯酚充當端粒酶的有效候選調節劑^[6]。2009年，有學者研究不同濃度的肝素對DLD-1細胞的影響，因為之前有硏究指出肝素對某些癌症的凋亡和抗增殖作用，然而研究結果最終表明肝素在體外對結腸癌細胞沒有明顯的抗增殖和凋亡作用^[7]。

參考資料

^ Chen, TR; Dorotinsky, CS; McGuire, LJ; Macy, ML; Hay, RJ. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin.. Cancer genetics and cytogenetics. 1995-06, 81 (2): 103–8 [2019-12-20]. PMID 7621404. doi:10.1016/0165-4608(94)00225-z. （原始內容存檔於2019-12-20）.
^ Okuyama, H.; Endo, H.; Akashika, T.; Kato, K.; Inoue, M. Downregulation of c-MYC Protein Levels Contributes to Cancer Cell Survival under Dual Deficiency of Oxygen and Glucose. Cancer Research. 2010-10-27, 70 (24): 10213–10223. doi:10.1158/0008-5472.CAN-10-2720.
^ Dou, Y; Cha, DJ; Franklin, JL; Higginbotham, JN; Jeppesen, DK; Weaver, AM; Prasad, N; Levy, S; Coffey, RJ; Patton, JG; Zhang, B. Circular RNAs are down-regulated in KRAS mutant colon cancer cells and can be transferred to exosomes.. Scientific reports. 2016-11-28, 6: 37982 [2019-12-20]. PMID 27892494. doi:10.1038/srep37982. （原始內容存檔於2019-12-20）.
^ Grabocka, E; Pylayeva-Gupta, Y; Jones, MJ; Lubkov, V; Yemanaberhan, E; Taylor, L; Jeng, HH; Bar-Sagi, D. Wild-type H- and N-Ras promote mutant K-Ras-driven tumorigenesis by modulating the DNA damage response.. Cancer cell. 2014-02-10, 25 (2): 243–56 [2019-12-20]. PMID 24525237. doi:10.1016/j.ccr.2014.01.005.
^ 林建賢.探討膽囊收縮素受體A在人類大腸癌細胞的表現量以及與細胞移動和侵襲能力的關聯性.中山醫學大學生化暨生物科技研究所學位論文.2016年
^ Eitsuka, T; Nakagawa, K; Miyazawa, T. Down-regulation of telomerase activity in DLD-1 human colorectal adenocarcinoma cells by tocotrienol.. Biochemical and biophysical research communications. 2006-09-15, 348 (1): 170–5 [2019-12-20]. PMID 16875674. doi:10.1016/j.bbrc.2006.07.029. （原始內容存檔於2019-12-20）.
^ Uzun, Y; Akdogan, E; Ozdemir, F; Ovali, E. The effects of heparin on DLD-1 colon cancer cell line.. Bratislavske lekarske listy. 2009, 110 (1): 3–6 [2019-12-20]. PMID 19408822. （原始內容存檔於2019-12-20）.

外部連結

Cellosaurus entry for DLD-1（頁面存檔備份，存於互聯網檔案館）

[1] Chen, TR; Dorotinsky, CS; McGuire, LJ; Macy, ML; Hay, RJ. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin.. Cancer genetics and cytogenetics. 1995-06, 81 (2): 103–8 [2019-12-20]. PMID 7621404. doi:10.1016/0165-4608(94)00225-z. （原始內容存檔於2019-12-20）.

[2] Okuyama, H.; Endo, H.; Akashika, T.; Kato, K.; Inoue, M. Downregulation of c-MYC Protein Levels Contributes to Cancer Cell Survival under Dual Deficiency of Oxygen and Glucose. Cancer Research. 2010-10-27, 70 (24): 10213–10223. doi:10.1158/0008-5472.CAN-10-2720.

[3] Dou, Y; Cha, DJ; Franklin, JL; Higginbotham, JN; Jeppesen, DK; Weaver, AM; Prasad, N; Levy, S; Coffey, RJ; Patton, JG; Zhang, B. Circular RNAs are down-regulated in KRAS mutant colon cancer cells and can be transferred to exosomes.. Scientific reports. 2016-11-28, 6: 37982 [2019-12-20]. PMID 27892494. doi:10.1038/srep37982. （原始內容存檔於2019-12-20）.

[4] Grabocka, E; Pylayeva-Gupta, Y; Jones, MJ; Lubkov, V; Yemanaberhan, E; Taylor, L; Jeng, HH; Bar-Sagi, D. Wild-type H- and N-Ras promote mutant K-Ras-driven tumorigenesis by modulating the DNA damage response.. Cancer cell. 2014-02-10, 25 (2): 243–56 [2019-12-20]. PMID 24525237. doi:10.1016/j.ccr.2014.01.005.

[5] 林建賢.探討膽囊收縮素受體A在人類大腸癌細胞的表現量以及與細胞移動和侵襲能力的關聯性.中山醫學大學生化暨生物科技研究所學位論文.2016年

[6] Eitsuka, T; Nakagawa, K; Miyazawa, T. Down-regulation of telomerase activity in DLD-1 human colorectal adenocarcinoma cells by tocotrienol.. Biochemical and biophysical research communications. 2006-09-15, 348 (1): 170–5 [2019-12-20]. PMID 16875674. doi:10.1016/j.bbrc.2006.07.029. （原始內容存檔於2019-12-20）.

[7] Uzun, Y; Akdogan, E; Ozdemir, F; Ovali, E. The effects of heparin on DLD-1 colon cancer cell line.. Bratislavske lekarske listy. 2009, 110 (1): 3–6 [2019-12-20]. PMID 19408822. （原始內容存檔於2019-12-20）.

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