花菁
花菁（英語：Cyanines，或譯為青色素、花青、青藍）是一大類熒光染料[1]，儘管其名字中帶「青」字，但其電磁波譜從紫外（UV）覆蓋的近紅外（NIR）。
| 探針 | 激發波長 | 發射波長 | 分子量 | 量子產率 |
|---|---|---|---|---|
| Cy2 | 489 | 506 | 714 | QY 0.12 |
| Cy3 | 550 | 570 | 767 | QY 0.15 [2] |
| Cy3B | 558 | 572 | 658 | QY 0.67 |
| Cy3.5 | 591 | 604 | 1102 | QY 0.15 |
| Cy5 | 646 | 662 | 792 | QY 0.27[2] |
| Cy5.5 | 675 | 694 | 1128 | QY 0.28[3] |
| Cy7 | 743 | 767 | 818 | QY 0.28 |
參考文獻
- ^ Kvach, Maksim V.; Ustinov, Alexey V.; Stepanova, Irina A.; Malakhov, Andrei D.; Skorobogatyi, Mikhail V.; Shmanai, Vadim V.; Korshun, Vladimir A. A Convenient Synthesis of Cyanine Dyes: Reagents for the Labeling of Biomolecules. European Journal of Organic Chemistry. 2008, 2008 (12): 2107–2117 [2022-11-02]. ISSN 1099-0690. doi:10.1002/ejoc.200701190. （原始內容存檔於2022-10-28） （英語）.
- ^ 2.0 2.1 Mujumdar B, Ernst A, Mujumdar SR, Lewis CJ, Waggoner AS. Cyanine dye labeling reagents: Sulfoindocyanine succinimidyl esters. Bioconjugate Chemistry. Mar 1993, 4 (2): 105–111. PMID 7873641. doi:10.1021/bc00020a001.
- ^ Umezawa K, Matsui A, Nakamura Y, Citterio D, Suzuki K. Bright, color-tunable fluorescent dyes in the Vis/NIR region: establishment of new "tailor-made" multicolor fluorophores based on borondipyrromethene. Chemistry. 2009, 15 (5): 1096–106. PMID 19117043. doi:10.1002/chem.200801906.