磷酸甘油酸脫氫酶
位於1號人類染色體的基因
磷酸甘油酸脫氫酶（英語：phosphoglycerate dehydrogenase，EC 1.1.1.95 （頁面存檔備份，存於互聯網檔案館）），是一種以NAD+或NADP+為受體、作用於供體CH-OH基團上的氧化還原酶。這種酶能催化以下兩種酶促反應：
| 磷酸甘油酸脫氫酶 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| |||||||||
| |||||||||
| 識別碼 | |||||||||
| EC編號 | 1.1.1.95 | ||||||||
| CAS號 | 9075-29-0 | ||||||||
| 數據庫 | |||||||||
| IntEnz | IntEnz瀏覽 | ||||||||
| BRENDA | BRENDA入口 | ||||||||
| ExPASy | NiceZyme瀏覽 | ||||||||
| KEGG | KEGG入口 | ||||||||
| MetaCyc | 代謝路徑 | ||||||||
| PRIAM | 概述 | ||||||||
| PDB | RCSB PDB PDBj PDBe PDBsum | ||||||||
| 基因本體 | AmiGO / EGO | ||||||||
| |||||||||
- 3-磷酸化-D-甘油酸 + NAD+ 3-磷酸羥基丙酮酸 + NADH + H+
- 2-羥基戊二酸 + NAD+ α-酮戊二酸 + NADH + H+
在大腸桿菌（Escherichia coli）中，磷酸甘油酸脫氫酶參與催化絲氨酸生物合成的磷酸絲氨酸途徑中的第一步，[1]上述可逆反應主要逆向進行。磷酸甘油酸脫氫酶可被絲氨酸或甘氨酸抑制。這種酶很不尋常，因為它還能像D-2-羥基戊二酸脫氫酶和L-2-羥基戊二酸脫氫酶一樣還原α-酮戊二酸，其中，D型底物更適合磷酸甘油酸脫氫酶。[2]
參考文獻
- ^ Pizer, L.I. The mechanism of end product inhibition of serine biosynthesis. I. Purification and kinetics of phosphoglycerate dehydrogenase. J. Biol. Chem. 1986, 243: 2081–2089. PMID 4384871.
- ^ Zhao, G. and Winkler, M.E. A novel α-ketoglutarate reductase activity of the serA-encoded 3-phosphoglycerate dehydrogenase of Escherichia coli K-12 and its possible implications for human 2-hydroxyglutaric aciduria. J. Bacteriol. 1996, 178: 232–239. PMID 8550422.
- Sugimoto, E. and Pizer, L.I. The mechanism of end product inhibition of serine biosynthesis. I. Purification and kinetics of phosphoglycerate dehydrogenase. J. Biol. Chem. 1968, 243: 2081–1089. PMID 4384871.
- Schuller, D.J., Grant, G.A. and Banaszak, L.J. The allosteric ligand site in the Vmax-type cooperative enzyme phosphoglycerate dehydrogenase. Nat. Struct. Biol. 1995, 2: 69–76. PMID 7719856.
擴展閱讀
- Tsang HT, Connell JW, Brown SE; et al. A systematic analysis of human CHMP protein interactions: additional MIT domain-containing proteins bind to multiple components of the human ESCRT III complex.. Genomics. 2006, 88 (3): 333–346. PMID 16730941. doi:10.1016/j.ygeno.2006.04.003.
- Gregory SG, Barlow KF, McLay KE; et al. The DNA sequence and biological annotation of human chromosome 1.. Nature. 2006, 441 (7091): 315–321. PMID 16710414. doi:10.1038/nature04727.
- Guo D, Han J, Adam BL; et al. Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress.. Biochem. Biophys. Res. Commun. 2005, 337 (4): 1308–18. PMID 16236267. doi:10.1016/j.bbrc.2005.09.191.
- Gerhard DS, Wagner L, Feingold EA; et al. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res. 2004, 14 (10B): 2121–2127. PMC 528928
. PMID 15489334. doi:10.1101/gr.2596504. - Ota T, Suzuki Y, Nishikawa T; et al. Complete sequencing and characterization of 21,243 full-length human cDNAs.. Nat. Genet. 2004, 36 (1): 40–45. PMID 14702039. doi:10.1038/ng1285.
- Strausberg RL, Feingold EA, Grouse LH; et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.. Proc. Natl. Acad. Sci. U.S.A. 2003, 99 (26): 16899–16903. PMC 139241 . PMID 12477932. doi:10.1073/pnas.242603899.
- Pind S, Slominski E, Mauthe J; et al. V490M, a common mutation in 3-phosphoglycerate dehydrogenase deficiency, causes enzyme deficiency by decreasing the yield of mature enzyme.. J. Biol. Chem. 2002, 277 (9): 7136–7143. PMID 11751922. doi:10.1074/jbc.M111419200.
- Klomp LW, de Koning TJ, Malingré HE; et al. Molecular characterization of 3-phosphoglycerate dehydrogenase deficiency--a neurometabolic disorder associated with reduced L-serine biosynthesis.. Am. J. Hum. Genet. 2001, 67 (6): 1389–1399. PMC 1287916 . PMID 11055895. doi:10.1086/316886.
- Pineda M, Vilaseca MA, Artuch R; et al. 3-phosphoglycerate dehydrogenase deficiency in a patient with West syndrome.. Developmental medicine and child neurology. 2000, 42 (9): 629–633. PMID 11034457. doi:10.1017/S0012162200001171.
- Baek JY, Jun DY, Taub D, Kim YH. Assignment of human 3-phosphoglycerate dehydrogenase (PHGDH) to human chromosome band 1p12 by fluorescence in situ hybridization.. Cytogenet. Cell Genet. 2000, 89 (1-2): 6–7. PMID 10894924. doi:10.1159/000015577.
- Cho HM, Jun DY, Bae MA; et al. Nucleotide sequence and differential expression of the human 3-phosphoglycerate dehydrogenase gene.. Gene. 2000, 245 (1): 193–201. PMID 10713460. doi:10.1016/S0378-1119(00)00009-3.
- Jaeken J, Detheux M, Van Maldergem L; et al. 3-Phosphoglycerate dehydrogenase deficiency: an inborn error of serine biosynthesis.. Arch. Dis. Child. 1996, 74 (6): 542–545. PMC 1511571 . PMID 8758134. doi:10.1136/adc.74.6.542.
