KB细胞

KB是一种被指属于学术丑闻的细胞系，由哈里·伊格尔（Harry Eagle）在1955年建立^[1]，那时候伊格尔也研究着海拉细胞^[2]。最初它被认为是一种人类口腔表皮样癌细胞系^[1]，但是后来通过同工酶、HeLa标记染色体和DNA指纹分析发现起源细胞已经被海拉细胞所污染，应为一种与人类乳突病毒相关的内子宫腺癌（papillomavirus-related endocervical adenocarcinoma）细胞系。 1966年，遗传学家斯坦利·加特勒（Stanley Gartler）发现这个事实后，翌年发表一篇名为《Genetic Markers as Tracers in Cell Culture》的文章^[3]。Gartler的发现已通过多种方法得到了多次确认。例如1976年，Nelson-Rees在《科学》上发表文章确实此事^[4]，又例如2001年Masters等作出的研究^[5]。

尽管已经确定KB细胞早已被海拉细胞所污染，替代了原始细胞，但是此后仍然有许多研究人员把KB细胞当作人类口腔表皮样癌细胞系，并且将其研究发表于期刊，或者以论文的方式发表。然而部分出版物将KB细胞正确地描述它是一种宫颈癌细胞系，或者称其为海拉细胞的衍生物。这可能提高对KB细胞作为错误识别细胞系的认识。2017年，Vaughan等在《Cancer Research》发表一篇文章，探究了错误识别KB细胞所造成的影响。他们指出2000年至2014年期间发表的631篇期刊文章中都使用了KB细胞，其中574篇文章错误描述KB细胞，仅有57篇文章正确描述KB细胞^[6]。

参考资料

^ ^1.0 ^1.1 EAGLE, H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB.. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 1955-07, 89 (3): 362–4 [2020-02-19]. PMID 13254761. doi:10.3181/00379727-89-21811. （原始内容存档于2020-02-19）.
^ EAGLE, H. The specific amino acid requirements of a human carcinoma cell (Stain HeLa) in tissue culture.. The Journal of experimental medicine. 1955-07-01, 102 (1): 37–48 [2020-02-19]. PMID 14392239. doi:10.1084/jem.102.1.37. （原始内容存档于2020-02-19）.
^ Gartler, SM. Genetic markers as tracers in cell culture.. National Cancer Institute monograph. 1967-09, 26: 167–95 [2020-02-19]. PMID 4864103. （原始内容存档于2020-02-19）.
^ Nelson-Rees, WA; Flandermeyer, RR. HeLa cultures defined.. Science (New York, N.Y.). 1976-01-09, 191 (4222): 96–8 [2020-02-16]. PMID 1246601. doi:10.1126/science.1246601. （原始内容存档于2020-02-16）.
^ Masters, JR; Thomson, JA; Daly-Burns, B; Reid, YA; Dirks, WG; Packer, P; Toji, LH; Ohno, T; Tanabe, H; Arlett, CF; Kelland, LR; Harrison, M; Virmani, A; Ward, TH; Ayres, KL; Debenham, PG. Short tandem repeat profiling provides an international reference standard for human cell lines.. Proceedings of the National Academy of Sciences of the United States of America. 2001-07-03, 98 (14): 8012–7 [2020-02-19]. PMID 11416159. doi:10.1073/pnas.121616198. （原始内容存档于2020-02-19）.
^ Vaughan, L; Glänzel, W; Korch, C; Capes-Davis, A. Widespread Use of Misidentified Cell Line KB (HeLa): Incorrect Attribution and Its Impact Revealed through Mining the Scientific Literature.. Cancer research. 2017-06-01, 77 (11): 2784–2788 [2020-02-19]. PMID 28455420. doi:10.1158/0008-5472.CAN-16-2258. （原始内容存档于2020-02-19）.

外部链接

Cellosaurus entry for KB （页面存档备份，存于互联网档案馆）

[EAGLE-1] 1.0 ^1.1 EAGLE, H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB.. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 1955-07, 89 (3): 362–4 [2020-02-19]. PMID 13254761. doi:10.3181/00379727-89-21811. （原始内容存档于2020-02-19）.

[2] EAGLE, H. The specific amino acid requirements of a human carcinoma cell (Stain HeLa) in tissue culture.. The Journal of experimental medicine. 1955-07-01, 102 (1): 37–48 [2020-02-19]. PMID 14392239. doi:10.1084/jem.102.1.37. （原始内容存档于2020-02-19）.

[3] Gartler, SM. Genetic markers as tracers in cell culture.. National Cancer Institute monograph. 1967-09, 26: 167–95 [2020-02-19]. PMID 4864103. （原始内容存档于2020-02-19）.

[4] Nelson-Rees, WA; Flandermeyer, RR. HeLa cultures defined.. Science (New York, N.Y.). 1976-01-09, 191 (4222): 96–8 [2020-02-16]. PMID 1246601. doi:10.1126/science.1246601. （原始内容存档于2020-02-16）.

[5] Masters, JR; Thomson, JA; Daly-Burns, B; Reid, YA; Dirks, WG; Packer, P; Toji, LH; Ohno, T; Tanabe, H; Arlett, CF; Kelland, LR; Harrison, M; Virmani, A; Ward, TH; Ayres, KL; Debenham, PG. Short tandem repeat profiling provides an international reference standard for human cell lines.. Proceedings of the National Academy of Sciences of the United States of America. 2001-07-03, 98 (14): 8012–7 [2020-02-19]. PMID 11416159. doi:10.1073/pnas.121616198. （原始内容存档于2020-02-19）.

[6] Vaughan, L; Glänzel, W; Korch, C; Capes-Davis, A. Widespread Use of Misidentified Cell Line KB (HeLa): Incorrect Attribution and Its Impact Revealed through Mining the Scientific Literature.. Cancer research. 2017-06-01, 77 (11): 2784–2788 [2020-02-19]. PMID 28455420. doi:10.1158/0008-5472.CAN-16-2258. （原始内容存档于2020-02-19）.

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